Supplementary material to Lang et al Searching the evolutionary origin of epithelial mucus protein components – mucins and FCGBP. Molecular Biology and Evolution 2016. doi:10.1093/molbev/msw066
For more on mucin sequences see also The mucin database.
Download sequences in fasta format
Tables of proteins, including sequence and domain structure
Protein domain structures (pdf)
Trees corresponding to Fig.1 but with bootstrap values (Newick format)
Tree corresponding to Figure 2 but based on PhyML with 100 bootstrap replicates. Branches without a number have bootstrap values = 100.
Predicted genomic structure of two mucin genes in M. leidyi
- Vertebrate proteins Vertebrate mucin domain structures classified as Muc2, Muc5ac, Muc5b, Muc6 and Muc19. Domain structures were predicted with hmmscan of the hmmer package (hmmer.janelia.org), using Pfam domains (Finn et al. 2014) and plotted with in-house R scripts. Proteins are taxonmically sorted and are shown for the groups Muc19, Muc6, Muc2, Muc5 and ovomucin.
- Vertebrate proteins predicted from genomic sequences Domain structures of vertebrate mucins predicted from genomic sequences. Sequences were predicted using genewise (Birney, Clamp, Durbin 2004) as described under Materials and Methods.
- X. tropicalis
- D. rerio
- Invertebrate proteins
- Invertebrate proteins predicted from genomic sequences Domain structures of invertebrate mucins predicted from genomic sequences. Sequences were predicted using genewise (Birney, Clamp, Durbin 2004)
- pdf The two mucin genes are on opposite strands as indicated. Vertical bars represent exons. Exons that contain VWD domains in red and PTS domains in blue.